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ScNCR1 has been localized to the yeast vacuole and T. Share this article Doi. Parasites were cloned by limiting dilution. In this study, we investigated a gene that acquired single nucleotide polymorphisms SNPs or was amplified in selections with three diverse compounds. With this strategy, PfNCR1 is expressed from its native promoter and contains a C-terminal green-fluorescent protein GFP tag in addition to the mutation. We used single-crossover allelic exchange to introduce one mutation from each resistance selection into a clean genetic background Figure 1—figure supplements 1 — 2. Here we show that PfNCR1 is druggable and necessary for maintaining the proper membrane lipid composition of blood-stage parasites. These cookies enable us to make the Website more relevant to your interests and to help us serve ads that might be of interest to you. Compound IDs matching with mutations are shown in lower case numbers and match Figure 1A. When we removed aTc from highly synchronized, young ring-stage parasites, PfNCR1 expression in trophozoites was reduced within the same cell cycle and undetectable in the following cell cycles, as judged by western blots to detect a C-terminal hemagglutinin HA sequence on the aptamer-tagged parasites Figure 2A. I am 18 or older - Enter I am under 18 - Exit. GraphPad Prism 5.
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